Qilian Huaji decoction exerts an anti-cancer effect on hepatocellular carcinoma by upregulating miR-122

Qilian Huaji Decoction (QLHJD) is a mixture of 13 herbs. The aim this study to observe the efficacy QLHJD against hepatocellular carcinoma (HCC). H22 transplanted mouse models were established explore effect on HCC in vivo. QLHJD-containing serum (QCS) was prepared using traditional Chinese medicine pharmacology methods. After QCS treatment, cell proliferation, cycle and apoptosis HepG2 cells assessed by MTT flow cytometry, respectively. miRNA expression profiles determined qPCR arrays. miR-122 examined tissue samples cells. AKT3 protein detected Western blot. We found that significantly inhibited growth tumor improve immune function In vitro, proliferation inducing arrest at S phase, induced altered reduced levels lines up-regulated compared with control group, accompanied decrease WNT1 expression. conclusion, could inhibit restoring aberrant miR-122, which may be one mechanisms whereby exerts its HCC.